Jan 15, 2012· Results. A method was developed for simultaneous quantification of multiple phytohormones, abscisic acid, indole-3-acetic acid (IAA), jasmonic acid (JA), and salicylic acid, hormone conjugates, IAA-aspartic acid, JA-isoleucine, and methyl JA, and phytoalexins, momilactone A, naringenin, and sakuranetin.

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concentration of 3.0 mg L-1 and 0.5 mg -L 1 respectively [10]. Micro-propagation is the practice in which aseptic conditions are adapted using modern plant tissue culture methods to produce a large number of progeny plants by rapidly multiplying stock plant material. Propagation of banana through in vitro techniques using different

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Jun 23, 2020· Phytohormones, such as auxins and cytokinins, take part in the integration of growth control and stress response, but their role in algal adaptation to …

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To qualitatively identify and quantify phytohormones, the plant material needs to be homogenized and extracted with suit-able solvents. The crude extract might contain interfering sub-stances from the plant matrix that require removal to obtain a sufficiently pure sample for analysis. Therefore, the choice of …

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Cryptic peptides (cryptides) are small bioactive molecules generated via degradation of functionally active proteins. Only a few examples of plant cryptides playing an important role in plant defense have been reported to date, hence our knowledge about cryptic signals hidden in protein structure remains very limited. Moreover, little is known about how stress conditions influence the size of ...

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Phytohormones are chemical messengers produced in one part of plant and translocated to the other parts, where they play critical roles in regulating plant responses to stress at extremely low concentration. Phytohormones are natural products and they called plant growth regulators, when they are synthesized chemically.

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Jul 21, 2018· Plants adjust their architecture by modulating organ growth. This ability is largely dependent on phytohormones. While responses to phytohormones have been studied extensively, it remains unclear to which extent and how these responses are modulated in non‐reference strains.

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Plant hormones have pivotal roles in plant growth, development and response to biotic and abiotic cues 1,2,3.Each class of hormones has characteristic biological effects.

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For a review of auxin determination methods, see my article Tivendale and Cohen (2015) J Plant Plant Growth Regul DOI: 10.1007/s00344-015-9519-4. Cite 8th Jan, 2016

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Jan 10, 2017· As integral parts of plant signaling networks, phytohormones are involved in the regulation of plant metabolism and growth under adverse environmental conditions, including salinity. Globally, salinity is one of the most severe abiotic stressors with an estimated 800 million hectares of arable land affected. Roots are the first plant organ to sense salinity in the soil, and are the initial ...

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This review examines contemporary views of the role of plant hormones in the control of physiological processes. Past and present difficulties with nomenclature encapsulate the problems inherent in using the 'classic' hormone concept in plants, with their distinctive multicellular organization.

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Jun 13, 2020· Quantification of endogenous hormones in plants is essential to understand their growth, development and response to biotic and abiotic stresses. Howe…

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TECHNIQUES FOR MOLECULAR ANALYSIS The use of vapor phase extraction in metabolic profiling of phytohormones and other metabolites Eric A. Schmelz1,*, Juergen Engelberth2, James H. Tumlinson2, Anna Block3 and Hans T. Alborn4 1Center of Medical, Agricultural, and Veterinary Entomology USDA†, Agricultural Research Service, 1600/1700 Southwest 23rd Drive, Gainesville, FL …

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We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.

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This is a short review of the classical and new, natural and synthetic plant hormones and growth regulators (phytohormones) and highlights some of their uses in plant tissue culture. Plant hormones rarely act alone, and for most processes— at least those that are observed at the organ level—many of these regulators have interacted in order to produce the final effect.

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ABSTRACT. The salinity has complex effects on the physiological, nutritional and hormonal interactions of plants. This study aimed to evaluate the mitigating action of exogenous application of different types and concentrations of phytohormones on Carica papaya under salt stress. The experiment was conducted in greenhouse, using a randomized block design with treatments formed from a 2 x 3 x 5 ...

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Phytohormones are known to play vital roles in the ability of plants to acclimatize to varying environments, by mediating growth, development, source/sink transitions and nutrient allocation.

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May 26, 2016· Plant metabolites are commonly functionally classified, as defense- or growth-related phytohormones, primary and specialized metabolites, and so forth. Analytical procedures for the quantifications of these metabolites are challenging because the metabolites can vary over several orders of magnitude in concentrations in the same tissues and have very different chemical characteristics.

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Addition of fresh medium to stationary cells of Arabidopsis suspension culture induces increased phosphorylation of the S6 ribosomal protein and activation of its cognate kinase, AtS6k. Analysis of the activation response revealed that medium constituents required for S6 kinase activation were the phytohormones 1-naphthylacetic acid (auxin) and kinetin. Pretreatment of cells with anti-auxin or ...

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The development and growth of plant organs is regulated by phytohormones, which constitute an important area of plant science. The last decade has seen a rapid increase in the unravelling of the pathways by which phytohormones exert their influence.

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Aug 23, 2016· Plant extracts were spiked with a low concentration (0.1 ng/μL) of individual phytohormones and extracted by following the Balcke et al. (2012) method for SPE elution. Figure 1 indicates the recovery of the spiked phytohormones from plant extracts in which no phytohormones were detected in the flow‐through eluate of the samples loaded onto ...

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Aug 16, 2004· The use of vapor phase extraction in metabolic profiling of phytohormones and other metabolites ... the stated goal of metabolomic analyses is to indiscriminately identify and quantify all plant metabolites in an effort to ... are useful for the concentration of acidic phytohormones and aid in the removal of major components including lipids ...

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Sep 02, 2003· Phytohormones regulate the protective responses of plants against both biotic and abiotic stresses by means of synergistic or antagonistic actions referred to as signaling crosstalk. A bottleneck in crosstalk research is the quantification of numerous interacting phytohormones and regulators. The chemical analysis of salicylic acid, jasmonic acid, indole-3-acetic acid, and abscisic …

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1. Introduction. Quantitative plant metabolomics is a tool that helps to improve our understanding of plant biochemistry and metabolism by delivering the accurate measurement, prior to statistical and bioinformatics analysis, of the concentrations of known metabolites that occur in different levels in plant samples [1,2].The two most commonly used analytical technologies driving quantitative ...

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water plants in Ohio during 23 regular and 3 QC sampling trips in 2008–10. Samples were collected at various stages in the treatment processes and analyzed for bacterial indicators : E. coli, fecal coliforms, and enterococci by membrane filtra-tion; somatic and F-specific coliphage by the single agar layer

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Dec 24, 2014· Plants are exposed to a variety of abiotic stresses in nature and exhibit unique and complex responses to these stresses depending on their degree of plasticity involving many morphological, cellular, anatomical, and physiological changes. Phytohormones are known to play vital roles in the ability of plants to acclimatize to varying environments, by mediating growth, …

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Aug 23, 2016· The different phytohormones were specifically quantified using precursor and product ions that were comparable to a previous report (Balcke et al., 2012).Using the MRM-MS method for quantification purposes of the seven phytohormones in a plant matrix permits a vital increase in sensitivity of the analytical method by reducing the noise level and contributing to the specificity of the …

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Plant materials and treatments. Plants of WT N. tabacum 'NC89' and phytochrome mutants were grown from seed, one per plastic pot (15 cm diameter×15 cm deep) containing the Pindstrup substrate (Pindstrup Mosebrug A/S), in a temperature-controlled growth chamber at an irradiance of 60 μmol m – 2 s – 1 on a 12 h light/12 h dark cycle and ...

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